Microbial Ecology & Eco Systems

Biography

Biography: Nikolaj Sørensen

Abstract

Traditionally, shotgun sequencing of complex microbial communities depends on reference genomes from known organisms. However, reference genomes are missing for the majority of the microbial diversity, making this approach inadequate for surveying complex microbial communities. The metagenomic species (MGS) concept allows detection and functional profiling of unknown species for which reference genomes are absent by clustering genes based on their abundance profiles. This method has already proven successful on the human gut microbiome, where it can identify up to five times more species than reference genomes can. The MGS concept has recently been expanded to include infants, mice, pigs, and marine microbial communities and can likely also be applied to terrestrial environments. Recent tests have found that the MGS concept is much more accurate and sensitive in detecting low-abundance taxa than a reference genome-based approach and, consequently, can provide reliable results at much lower sequencing depths, providing significant savings on sequencing costs. The MGSs typically consists of 1000-2000 genes representing core genomes and annotation of these genes allows identification of the functional potential of each MGS as well as insight into how metabolic pathways are distributed between the members of a microbial community. Furthermore, the majority of the MGSs appears to be only distantly related to known taxa and can provide a vantage point from which to describe new high level taxa. The MGS concept is a very valuable tool for the microbial ecologist and allows surveying the microbial community at both a taxonomic and functional level of detail not previously possible.